Chromatography AKTA FLPC

Major geek post, today.  Turn back while you still can!

I have to go into the lab today AND go work at the bookstore.  I love both of my jobs, but it’s Saturday…ewwww.  Internet fun…fresh air bad (well, in Minnesota in January, anyway).

At my job I sometimes do FPLC, which is a way cool chemistry tool for separating and purifying proteins.  But it can get pretty confusing very quickly.  There are all sorts of inputs and outputs, and sometimes I get mixed up with the tubes going all over the place:

“I fought the AKTA, and the AKTA won!”
FPLC image – http://psf.cobre.ku.edu
Stickman was added by biodork.

And sometimes the instrument acts up and and I get so fed up with it that I feel like:

FPLC image –  http://faculty.ksu.edu.sa/
T-Shirt image – http://www.hipstertees.co.uk/

There is also a way to make sure the columns (part of the set-up) are working well.  This method is called measuring “theoretical  plates“.  I imagine theoretical plates look something like this:

Plates image – http://images.crateandbarrel.com
Text added by biodork.


or maybe more like this:

Thought bubble image – http://www.polyvore.com
 Plates image – http: http://www.washingtonspaces.com

 

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